Use of phospholipid fatty acid (PLFA) analysis to describe microbial communities in soil

First Paragraph: It is far more complex to count and identify microorganisms than it is nematodes, earthworms and other soil organisms. We need reliable ways to measure microbial populations that:
i) are responsible of who is there
ii) provide useful information on type (species, trophic groups) and their metabolic status
iii)are not subject to interference from soil particles